Abstract
The fungus Irpex Lacteus is effective in decolourising methylene blue, a common dye in printing and dyeing wastewater, and it is of clear significance to investigate its optimal decolourisation process through liquid fermentation. The medium mixed with methylene blue dye was incubated with Irpex Lacteus in a constant temperature shaking flask liquid fermentation equipment, and single factors such as incubation time, temperature, shaking bed speed, dye concentration, pH, carbon and nitrogen sources were screened, and key factors were targeted orthogonal tests were carried out to optimise the best decolourisation process for Irpex Lacteus against methylene blue. The single-factor test revealed that carbon, nitrogen, pH and inoculum were the main factors influencing the decolourisation of methylene blue by Irpex Lacteus. The results of the orthogonal test showed that the decolourisation effect was best when the carbon source was sucrose, the nitrogen source was peptone, pH=3.5, the inoculum level was 7% and the incubation time was 10 days. Irpex Lacteus showed good decolourisation effect on methylene blue and the optimised process conditions provided a new direction for the treatment of printing and dyeing wastewater.
Highlights
China is a large textile printing and dyeing country, and the huge population is driving the rapid development of the textile manufacturing industry
After a certain number of days of incubation, the medium was centrifuged at 8000 r/min for 10 min, and the supernatant was taken and the absorbance was measured at 660 nm using a UV spectrophotometer, and the decolourisation rate was calculated according to the following formula: Decolorization rate =)A0-AC(/A0 ×100% (A0: Absorbance of the dye medium in the blank group, AC: Absorbance of dye media cultured after inoculation)
The nutrients and culture conditions of the fermentation medium are the main factors affecting the decolorization of Irpex Lacteus, the optimization of the nutrients and culture conditions of the Irpex Lacteus medium is one of the main routes to improve the decolorization of Irpex Lacteus,the decolorization rate of Irpex Lacteus could reach about 80% with sucrose as the carbon source and about 90% with peptone as the nitrogen source. pH is a parameter that requires critical control during fungal growth and fermentation and has an important impact on the decolorization method for non-fungal species [4], with optimal growth under acidic conditions, which has a key impact on methylene blue decolorization
Summary
China is a large textile printing and dyeing country, and the huge population is driving the rapid development of the textile manufacturing industry. The consequent problems of dyeing and printing wastewater treatment are becoming increasingly prominent, seriously affecting the surrounding environment, while the huge amount of money consumed for wastewater treatment is restricting the pace of development of large industries [1]. The current treatment methods for printing and dyeing wastewater include chemical, biological and physical methods, of which the biological method has been highly respected in recent years for its resource-saving, excellent effect and non-secondary pollution characteristics [2]. Irpex Lacteus Fr. is a rare medicinal fungus belong to the family polyporaceae, which is produced exclusively deep in the dense forests of Changbai Mountain in Jilin Province, and has good immunomodulatory effects, it is a fungus with biodegradability for dye wastewater pollution [3]. The study was conducted to investigate the optimal decolorization conditions (carbon source, nitrogen source,) and environmental conditions (inoculum, dye concentration, incubation time, incubation temperature, pH) for methylene blue using the biofermentation model of Irpex Lacteus
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