Study on the culture of Escherichia coli with different hydrolysis depth of globin

Zurong Song,Feng Xuehua,Ali Tao,X Wei

doi:10.1051/e3sconf/202021301001

Abstract

Objective: To explore the effect of culture medium made of peptide fragments of different sizes of globin hydrolyzed by protease on the culture of Escherichia coli. Method: with fresh pig blood as raw material, hydrolyze pig blood protein under fixed conditions, and screen out the enzyme with the highest degree of hydrolysis. Then conduct orthogonal test on the enzyme to determine the best hydrolysis conditions; the beads were obtained under the best hydrolysis conditions. The Escherichia coli was cultured on different peptone culture bases that were prepared by hydrolysate going through different pore size ultrafiltration membrane samples. The culture of Escherichia coli at different hydrolysis depths is determined by detecting the total amount with Escherichia coli detection method. Results: Trypsin has the best hydrolysis effect. The best hydrolysis conditions are as follows: enzyme dosage of 0.2%, hydrolysis time of 12h, temperature of 50°C, initial pH of 7.5 and mass fraction of substrate protein of 8%. Through growth curves of Escherichia coli, it is known that the smaller the pore size of the ultrafiltration membrane, the smaller the molecular weight of the peptide fragment, and the greater the density of the Escherichia coli bacterial solution. Conclusion: Through analyzing the production curves of Escherichia coli in different culture medium, it is concluded that the smaller the molecular weight of the protein peptides, the higher the density of Escherichia coli solution in the LB liquid medium prepared by it, and the better the Escherichia coli culture effect.

Highlights

In the processing and utilization of pig blood, except that a small part is used for food addition, most of it is discharged as waste, which pollutes the environment, and wastes protein resources[1]
The peptone contains a lot of amino acids, and more vitamins and other growth factors, which are extremely beneficial to the growth of bacteria such as Escherichia coli and can be used to provide basic raw materials, nitrogen sources, vitamins and other nutrients for the culture of it, which improves the utilization rate of pig blood resources, and accelerate the progress of Escherichia coli culture research[4,5,6]
With single factor and orthogonal experiments, it is obtained that the optimal hydrolysis conditions are: initial pH of 7.5, hydrolysis time of 12h, hydrolysis temperature of 50°C, enzyme dosage of 0.2%, and mass fraction of substrate protein of 8%

Summary

Introduction

In the processing and utilization of pig blood, except that a small part is used for food addition, most of it is discharged as waste, which pollutes the environment, and wastes protein resources[1]. We need to make use of pig blood, especially fresh pig blood. It can be decomposed into globin and other substances, and made into polypeptides needed in the food and pharmaceutical industries through hydrolysis reaction. The peptone contains a lot of amino acids, and more vitamins and other growth factors, which are extremely beneficial to the growth of bacteria such as Escherichia coli and can be used to provide basic raw materials, nitrogen sources, vitamins and other nutrients for the culture of it, which improves the utilization rate of pig blood resources, and accelerate the progress of Escherichia coli culture research[4,5,6].

Methods

Results

Conclusion