Abstract

Eggshell provides a relatively independent and stable internal environment for embryo growth and development, and is also a source of calcium ions required for embryo development. Eggshells of good quality can reduce the risk of food safety to a certain extent, and can also reduce the economic losses caused by broken eggshells and improve economic efficiency. At the same time, the process of eggshell formation is also a classic biomineralization model. In this study, the 600K high-density SNP chip of chicken was used to perform SNP typing on several groups obtained by the reciprocal cross between Bailaihang and Dongxiang green-shell hens. Scanning electron microscopy and X-ray crystal diffraction were used to determine the ultrastructure and eggshell ultrastructure. Crystal structure, explain its genetic basis, and explore the relationship between eggshell ultrastructure and crystal structure. This study believes that the DERA gene plays an important role in regulating the growth of eggshell crystals. There are three possible ways of action: one is to provide energy for the synthesis of uterine tissue cell matrix protein and its transport with ions; the second is to reduce the stress of high Ca2+ concentration stress, maintain cell activity, and for the growth of eggshell crystals Stably provide the required Ca2+; third, by regulating the concentration of ATP in the uterine fluid, the effect on the growth of eggshell crystals.

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