Abstract
In the study, three aristololactams were isolated from Piper wallichii and determined simultaneously by a HPLC method using a C 18 column with a gradient elution of methanol and water. The content ranges of the target compounds in twelve batches of Piper wallichii were 0.100-0.110 mg/g dry weight (cepharanone B), 0.198-0.208 mg/g dry weight (aristolactam AII), and 0.047-0.059 mg/g dry weight (aristololactam AIIIa), respectively. Their anti-proliferative effects in HK-2 cells were determined by MTT assay and cells membrane damage were assessed by LDH release assay. Results indicated that the test compounds showed a time- and dose-dependent cytotoxicity in HK-2 cells, and the values of IC 50 were cepharanone B > aristolactam AII > aristololactam AIIIa.
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