Study on the Characterization and Controlled Drug Release Carrier of Thiolated Chitosan-Based Nanoparticles

Abstract

In this paper, we prepared and characterized the thiolated chitosan nanoparticles (CS NPs) Through modification. Bridged with a carbodiimide reagent, L-cysteine was therefore covalently bound to CS NPs which are capable of forming double bonds with thiol group and the mucus glycoprotein. A quantitative analysis of the widely used Ellman’s method can obtain the amount of thiol groups in various compounds. The resulted polymer conjugation displayed that the amount of thiol groups attached onto CS NPs, was increased with increasing concentration of cysteine. The method C exhibited greater conjugation ratio of thiol groups attached onto CS NPs. In particular, the evidence of thiol groups attached onto CS NPs was present in the thiolated specimens, can be shown by FTIR spectra.