Abstract
Submerged fermentation by Phellinus igniarius was done using solid wastes of spent mushroom substrate and corn cob in the medium. On the basis of single factor experiments, orthogonal test with four factors and three levels was carried out using contents of flavonoids, soluble protein and mycelia biomass in fermentation broth as main reference indexes. The results showed the optimal formula was 0.5% yeast extract, 0.2% KH2PO4, and 0.1% MgSO4, when the ratio of wheat bran and the mix of spent mushroom substrate and corn cob (1:1) was 2:8, On the basis of optimum medium formula, three groups of parallel tests were carried out, the contents of flavonoids and soluble protein, mycelia biomass obtained were 0.465 mg/mL, 0.278 mu g/mL, and 1.378 g/100 mL respectively.
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