Abstract
A horseradish peroxidase (HRP)-Au nanoclusters (AuNCs) bionanocomposite was prepared simply by mixing HRP and NaAuCl4 in an alkaline aqueous solution (pH12) at 37°C for 12h under vigorous stirring. Cast-coating the HRP-AuNCs bionanocomposite and then Nafion on a glassy carbon electrode (GCE) yielded a Nafion/HRP-AuNCs/GCE. This electrode exhibited a pair of well-defined cyclic voltammetric peaks of the immobilized HRP with a formal potential of −0.389V vs SCE and an electron transfer rate constant of 3.19s−1 in pH7.0 phosphate buffer. The HRP also exhibits a high bioactivity. This electrode showed high electrocatalytic activity for reduction of both O2 and H2O2, as a result of the synergistic effects of HRP and nearby AuNCs. This electrode gave a linear amperometric response to H2O2 from 0.01 to 3.27mmolL−1 with a sensitivity of 0.86μAmM−1cm−2 and a limit of detection (LOD, S/N=3) of 0.99μM.
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