Study on the auxin-like activity of organic compounds extracted from corn waste hydrochar prepared by hydrothermal carbonization

Abstract

ABSTRACT This work studied the auxin-like activity of liquid and solid hydrochar from aboveground corn biomass prepared using hydrothermal carbonization (HTC). Understanding the action of organic compounds in regulating plant metabolism is important to develop strategies to improve plant growth and production. Bioassays were performed by testing liquid hydrochar concentrations in the range of 0.0557-5570.0 mg carbon L−1; and solid hydrochar (via extracted dissolved organic matter, DOM) in the range of 0.026-2600.0 mg carbon L−1, using seeds of Lactuca sativa. SEM, ATR-FTIR, and Py-GC/MS were applied to assess the effect of HTC on hydrochar production/composition. Liquid hydrochar presented an intense bioactivity, completely inhibiting the germination of testing seeds at higher concentrations. Liquid hydrochar also was considerably more bioactive. Py-GC/MS allowed the identification of the molecules involved in IAA-like effects: carboxylic acids (linear and aromatic) and amino acids. The concentration of more bioactive molecules, rather than their simple presence in the hydrochar fraction, determined the bio-stimulating effect, besides an excellent linear regression between the auxin-like effect and the concentration of active molecules.