Abstract
Ixora duffii is an important traditional medicinal plant in Vietnam. In this study its antioxidant property was investigated by diphenylpicrylhydrazyl (DPPH) radical and Fe3+ reducing assay and cytotoxic activity was evaluated against HepG2 cell line by MTT assay. The results showed that the methanolic extract of Ixora duffii flowers had DPPH radical scavenging activities similar to that of leaves extract. At the concentration of 100 g/mL, the extracts of flowers and leaves scavenged about 80% DPPH radical. The DPPH scavenging activity of Ixora duffii was lower than that of vitamin C approximately 2 times. The activity of Fe3+ reducing of flowers was higher than that of leaves, with EC50 values of 162.03 and 218.87 g/mL, respectively. Results were compared with the standard butylated hydroxyanisole (BHA) that was lower than 4.78 times in flowers and 6.76 times in leaves. The methanolic extract of Ixora duffii displayed significantly dose dependent in reducing the growth of HepG2, with 56% growth inhibitory concentration in a dose of 500 g/mL. The qualitative analysis of phytochemical compounds showed the presence of alkaloids, flavonoids, anthraquinones, terpenoids, quinones, glycoside, coumarins and phenols in the leaves and flower extracts of Ixora duffii. Compounds of tanins and saponins were only present in flowers of Ixora duffii. Total phenolic content were found in flowers (762.37 mg GAE/g) that was higher than the one in leaves (360.85 mg GAE/g). Flower and leave extracts exhibited a similar total flavonoid content of 679.55 mg QE/g and 676.35 mg QE/g, respectively.
Highlights
For their protective and anti-oxidant potentials and correlation with analytical data, Int
In this study its antioxidant property was investigated by diphenylpicrylhydrazyl (DPPH) radical and Fe3+ reducing assay and cytotoxic activity was evaluated against HepG2 cell line by MTT assay
The results showed that the methanolic extract of Ixora duffii flowers had DPPH radical scavenging activities similar to that of leaves extract
Summary
Ở Việt Nam, cây Trang To (Ixora duffii) phân năng khử gốc tự do Fe3+ (khử sắt) cho thấy hoa có bố phổ biến. Kết quả cho thấy hoa và lá cây Trang To Hàm lượng polyphenol tổng (TPC) ở hoa cây có khả năng trung hòa gốc tự do DPPH tương ứng Trang To (762,37±62,58 mg GAE/g) cao hơn ở lá với hiệu suất 81,41±1,21 % và 80,46±0,80 % ở (360,85±10,09 mg GAE/g) là 2,1 lần. Riêng loài Trang To (Ixora duffii) cũng thuộc chi Ixora nhưng vẫn chưa có nghiên cứu cụ thể nào về thành phần hóa học, khả năng kháng oxy hóa và gây độc tế bào ung thư của loài cây này. Mục tiêu nghiên cứu của đề tài này là khảo sát thành phần hóa học và khả năng kháng oxy hóa của cây Trang To (Ixora duffii) thuộc họ Cà phê (Rubiaceae) để bổ sung cơ sở khoa học về nguồn dược liệu có triển vọng tạo ra các sản phẩm phòng ngừa và điều trị một số bệnh mãn tính ở người
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