Abstract
Due to the excellent antioxidant capacity of proanthocyanidins, combined with the antioxidant mechanism of anthocyanins, it inspired us to study the antioxidant capacity of proanthocyanidins and their complexes. In this article, we conducted antioxidant tests on proanthocyanidin-bovine serum albumin and procyanidin-metal complexes, using DPPH and ABTS methods to determine the antioxidant capacity of procyanidins and their complexes. The results show that compared with proanthocyanidins, the proanthocyanidin complex has a certain degree of improvement in the ability to scavenge free radicals. After being combined with metal ions, the ability to scavenge free radicals is significantly improved. When the ratio of proanthocyanidin B2 to Fe3+ is 2:1, the ability to scavenge free radicals is significantly enhanced.
Highlights
Due to the excellent antioxidant capacity of proanthocyanidins, combined with the antioxidant mechanism of anthocyanins, it inspired us to study the antioxidant capacity of proanthocyanidins and their complexes
Add sequence according to different samples, the experimental results obtained in parallel 2 groups are: Under the corresponding concentration, the clearance rate of proanthocyanidin in absolute ethanol solution is 91.95%, 91.35%, 92.80%, 92.65% and 93.30%
According to the sample volume of the proanthocyanidin standard solution, the mass concentrations of 0.1 mg/mL, 0.2 mg/mL, 0.3 mg/mL, 0.4 mg/mL and 0.5 mg/mL can be obtained at different pH values
Summary
According to the sample amount of proanthocyanidin standard solution , the test solutions with mass concentrations of 0.1 mg/mL, 0.2 mg/mL, 0.3 mg/mL, 0.4 mg/mL and 0.5 mg/mL can be obtained. Add sequence according to different samples, the experimental results obtained in parallel 2 groups are: Under the corresponding concentration, the clearance rate of proanthocyanidin in absolute ethanol solution is 91.95%, 91.35%, 92.80%, 92.65% and 93.30%. 2.1.2 Results of the ability of proanthocyanidin samples to remove DPPH under different pH values. According to the sample volume of the proanthocyanidin standard solution , the mass concentrations of 0.1 mg/mL, 0.2 mg/mL, 0.3 mg/mL, 0.4 mg/mL and 0.5 mg/mL can be obtained at different pH values.
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