Study on the anti-Helicobacter pylori infection effects of berberine in mice

Abstract

Objective To observe the anti-Helicobacter pylori(Hp) infection effects of berberine in mice. Methods A young rats model of Hp infection was established, then the rats were divided into 3 groups according to different anti-Hp treatments.The standard group was given a standard triple therapy(amoxilin+ clasamycin+ omeprazole), the berberine group was added with berberine on the basis of the standard triple therapy; and the bismuth group was added with bismuth on the basis of the standard triple therapy.All the three groups received continuous treatment for 2 weeks.The eradication condition of Hp was detected by gastric mucosa Giemsa staining and rapid urinary enzyme test 4 weeks after discontinuation.In addition, the minimum antibacterial concentration of berberine was detected by using agar dilution method antibiotic sensitivity test through Hp culturing in vitro. Results The Hp eradication rate of standard triplet group, berberine group and bismuth group was 55.0%, 77.5% and 95.0%, repectively, the difference was statistically significant(P<0.05). The eradication rate of berberine group was higher than that of standard triplet group, but lower than that of bismuth group(P<0.05). Meanwhile, the minimum antibacterial concentration of berberine was 12.5 mg/ml in vitro. Conclusion Berberine has antibacterial ability against Hp, which combined with standard triple therapy can improve the eradication rate of Hp. Key words: Helicobacter pylori; Berberine; Minimum antibacterial concentration; Amoxilin; Clasamycin