Abstract
The development of a portable and inexpensive surface plasmon resonance (SPR) measurement device with the integrated biosensor for the detection of snake venom protein is presented in this paper. For the construction of the sensing element, amine coupling chemistry is used to bio-functionalize silver coated glass slide with antibodies like immunoglobulin (IgG). The immobilization of the antibody is confirmed by spectroscopic measurements like ultraviolet-visible spectroscopy (UV-Vis) and Fourier-transforms infrared spectroscopy (FTIR). The device is calibrated with the standard solution of sodium chloride and ethanol before testing venom protein samples. To investigate the bio-molecular interactions, crude venom of Indian cobra (concentration range: 0.1 mg/ml‒1.0 mg/ml) in the phosphate buffer solution (PBS) are exposed to the biosensor. The experimentally measured data indicate the shift in the plasmon resonance angle from its initial value (52°) to 54° for 0.1 mg/ml and 60° for 1.0 mg/ml protein solution.
Highlights
Optical biosensors have been used extensively in the scientific community for several purposes, most notably to determine the association and dissociation kinetics, protein-protein [1], or nucleic acid hybridization interactions [2], etc
We report on the design and fabrication of a surface plasmon resonance biosensor for the detection of snake venom protein using portable instruments in a cost effective way
The absorbance peak is observed at 310 nm, and the successive deposition of the overlying layer on the silver film results in a subsequent shift in the surface plasmon resonance (SPR) dip
Summary
Optical biosensors have been used extensively in the scientific community for several purposes, most notably to determine the association and dissociation kinetics, protein-protein [1], or nucleic acid hybridization interactions [2], etc. Since the demonstration of utility of surface plasmon resonance (SPR) as the optical biosensor [3], an increasing popularity of the technique is observed in fundamental biological studies, health care research, drug discovery and clinical diagnosis [4], environmental monitoring [5], food safety and security [6], and agricultural research, etc. SPR has been established as a potential tool for the detection of bio-analytes producing extraordinary detection limit and has added a unique advantage of real-time label-free detection of biological species in the molecular level. The traditional methods for the excitation of surface plasmon using methods like Kretschmann’s [14] and Otto’s [15] configurations
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
