Study on Seed Protein and Protein Profile Pattern of Chickpea (Cice arietinum L.) by SDS-PAGE under Drought Stress and Fertilization

Abstract

A field experiment was performed in order to evaluate the effects of drought stress and application of starter nitrogen fertilizer on seed storage proteins and protein pattern in chickpea cultivars. Experiment was performed in split-factorial using randomized complete block design with three replications. The experiment was laid out in a split-factorial design with drought stress in main plots and cultivar with nitrogen fertilizer in subplots with three replications. The experimental treatments consisted of three levels of drought stress [severe drought stress (S2), moderate drought stress (S1) and no drought stress(S0)] and two cultivars of chickpea consist of Azad and Bivanij and 2 N levels. Plants were either not given any N fertilizer (N0), or fertilized by N fertilizer at the rate of 25 kg ha-1 (N25). The results of this study showed that the effects of drought stress on seed storage proteins and protein yield, effect of cultivars on protein yield were significant. With increase of drought stress seed storage proteins was increased and protein yield decreased. Severe drought stress treatment has the highest seed protein and the control treatment has the lowest seed protein. Non stress treatment has the highest protein yield and the severe drought stress treatment has the lowest protein yield. The SDS- PAGE results revealed that no effects treatments on the protein banding patterns but the related severe drought stress bands were chromatic, because they have highest protein concentration in some protein bands.DOI: http://dx.doi.org/10.3126/ijls.v9i5.12704