Abstract
This paper mainly examined the anti-osteosarcoma activity of total flavonoids extract from traditional Chinese medicine, nidus vespae. Orthogonal design was used to design the extraction process of total flavonoids. L(3(4)) orthogonal test was performed and the extracts obtained by three optimal extraction processes were used for anti-tumour activity screening in order to determine the optimal anti-tumour effective component of nidus vespae. MTT assay was used to investigate the effect of nidus vespae extract on proliferation activity of osteosarcoma cells. Meanwhile, U2OS cell inhibitory capacities of extracts in three groups with higher total flavonoid contents were investigated and compared, and inhibition rates were calculated. The results showed that the optimal extraction process was ethanol concentration of 95%, 12-fold amount of ethanol relative to the weight of medicinal material, extraction times of 3 times, and extraction time of 2 hours. 9 extraction processes all showed proportional trend of cancer cell inhibition rate to extract concentration.
Highlights
Trypanosomosis is a fatal disease of human and domestic animals in tropical Africa and South America spread by the bite of an infected tsetse fly (Glossina Genus) (Fairlamb, 1982)
In furtherance of the efforts on sourcing for novel molecules from natural sources in combating African animal trypanosomosis, previous research on in vivo antitrypanosomal activity is very limited compared to the in vitro experiments, and in the few in vivo studies found in literature, no complete cure without relapses was recorded for plants such as Alstonia boonei bark, Annona senegalensis root, Morinda lucida leaves and Picralima nitida (Wosu and Ibe, 1989)
Since infection models in mice have indicated that African trypanosomosis trigger anti-inflammatory responses (Namangala et al, 2001), the most active partitioned fraction was evaluated for its antiinflammatory response(s) in mice
Summary
Trypanosomosis is a fatal disease of human and domestic animals in tropical Africa and South America spread by the bite of an infected tsetse fly (Glossina Genus) (Fairlamb, 1982). It has undergone a dramatic and devastating resurgence within the last two decades (Smith et al, 1998), especially in Sub-Saharan Africa (Welburn et al, 2001). In furtherance of the efforts on sourcing for novel molecules from natural sources in combating African animal trypanosomosis, previous research on in vivo antitrypanosomal activity is very limited compared to the in vitro experiments, and in the few in vivo studies found in literature, no complete cure without relapses was recorded for plants such as Alstonia boonei bark, Annona senegalensis root, Morinda lucida leaves and Picralima nitida (Wosu and Ibe, 1989). Since infection models in mice have indicated that African trypanosomosis trigger anti-inflammatory responses (Namangala et al, 2001), the most active partitioned fraction was evaluated for its antiinflammatory response(s) in mice
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