Study on release of cardamom extract as an antibacterial agent from electrospun scaffold based on sodium alginate

Abstract

In the present study, sodium alginate (SA):polyvinyl alcohol(PVA) blends with different concentrations of cardamom extract (CE) were electrospun. The SEM images show that the optimum content of CE was 10 wt% resulting in fibers with average diameter of 233 ± 33 nm. FTIR spectra was employed to investigate probable interactions between polymers and CE. The CE release follows first order kinetics with 86% initial burst release at the first 24 h necessary to desired antibacterial activity. To investigate the biological properties, MTT assay, cell adhesion and antibacterial test were carried out. The SA:PVA:CE nanofibers showed no toxicity against fibroblast cells. The cell culture results exhibited the proper biocompatibility and proliferation on both CE-loaded and unloaded webs. The antibacterial test showed that CE-loaded nanofibrous web is capable of improving the antibacterial activity about 99% and 97% against gram-positive and gram-negative bacteria, respectively. Therefore, the CE-loaded web can be used as an antimicrobial wound dressing for healing improvement.