Study on relationshipbetween serum endothelin -1.NO and hemodynamics and liver function in pig orthotopic livertransplantation.

Abstract

：Objective To investigatethe relationship between changes of serum endothelin-1 (ET-1) and nitric oxide (NO) andchanges in hemodynamics and liver function in pigs with orthotopic livertransplantation(OLTx). Methods 38 healthy pigs were set at random with 19 donators and 19receivers. The hemodynamic changes were continuously monitored during the operatingphases. The serum ALT, AST, TBIL, ALB and ET-1, NO levels before operation, duringanhepatic phase, at 5 and 30 minutes after reperfusion, and at operation end weremeasured. Results The serum ET-1 and NO levels and the ratio of ET-1/NO increasedobviously and reached the peak values at 30 mins after reperfusion. At 30 mins afterreperfusion, the level of MAP was positively correlated with the serum ET-1 and ratio ofET-l/NO. At 5 mins after reperfusion, the level of MAP and CVP were negatively correlatedwith the serum NO. At 30 mins after reperfusion, the level of ALT was positivelycorrelated with the serum ET-1 and ratio of ET-l/NO. Conclusion The imbalance of serumET-1 and NO may be important mechanism leading to hemodynamics changes due to graft. Theserum ET-1 may cause hypoxic damage of hepatocyte. NO may improve microcirculation ofliver, and play a protective reperfusion in OLTs.