Study on related factors in airway bacterial culture and inner tubes disinfection methods among patients after trachenstomy

Abstract

Objective To investigate the classification of major bacteria and pathogen strains before and after inner tubes disinfection within the trachea after the tracheotomy, and to compare the method of disinfection and the results. Methods 110 patients after tracheotomy were randomly selected, and tracheal tubes were sampled before the disinfection and were conducted a bacterial culture analysis. These tracheal tubes were disinfected by boiling for 20 minutes and 30 minutes, respectively. Then they were soaked in 75% ethanol, 2% glularaldehyde and 3% hydrogen peroxide for 20 minutes and 30 minutes, respectively. The disinfection effects were compared among the four disinfection methods and disinfectants, and the two groups of disinfection time. Results 62 cases before the disinfection were sampled to make a bacterial culture. 89 strains in 18 species were cultivated, and no bacteria in six cases. Pathogenic bacteria and conditioned pathogen accounted for 80 percent (P<0.05). For the four disinfection methods including boiling disinfection, 75% ethanol, 2% glutaraldehyde and 3% hydrogen peroxide immersion disinfection, after 20 minutes of disinfection and immersion disinfection, 75% ethanol immersion disinfection had statistical meanings with the positive rates of boiling disinfection, 3% H2O2 and 2% glutaraldehyde soaking disinfection, respectively (P<0.05) ; After 30 minutes of disinfection, all of the four disinfection methods had no statistical meaning. Conclusions Pathogenic bacteria cultivated before the disinfection of tracheal tubes are much more than the non-pathogenic bacteria. Boiling disinfection for 20 minutes, 3% hydrogen peroxide and 2% glutaraldehyde disinfectant soaking for 20 minutes, and 75% ethanol immersion disinfection for 30 minutes can achieve good disinfection results. From the aspects of high efficiency, safety and low cost, 3% hydrogen peroxide is the best choice. Key words: Tracheostomy; Bacterial culture; Inner tubes; Disinfection