Abstract
Optical clearing agents can improve tissue optical transmittance by reducing the diffuse reflection. The reflection on in vivo human skin before and after applying anhydrous glycerol and 30 to 50% liquid paraffin glycerol mixed solution are investigated in this paper. From their visible and near-infrared reflection spectroscopy, all of their diffuse reflections are reduced after applying the agents. It is found that the three mixed solutions show stronger effect than that of anhydrous glycerol. These results further prove liquid paraffin can enhance the percutaneous penetration of glycerol and take synergistically optical clearing effect with glycerol over visible and near-infrared wave bands.
Highlights
Motivated by the growing maturity of laser treatment and optical imaging diagnosis, optical techniques, such as optical coherence tomography (OCT), confocal microscopy, nonlinear microscopy, and laser spectroscopic methods, are widely used in many fields
Four kinds of solution were used in this study, including anhydrous glycerol and three different concentrations of liquid paraffin whose volume fractions in mixed solutions are 30, 40, and 50%, respectively
It can be seen that the results from all four agents have similar trends qualitatively; that is, the diffuse reflection decreases gradually with time elapsing over the whole wavelength range investigated
Summary
Motivated by the growing maturity of laser treatment and optical imaging diagnosis, optical techniques, such as optical coherence tomography (OCT), confocal microscopy, nonlinear microscopy, and laser spectroscopic methods, are widely used in many fields. The complicated morphological nature of human tissue and variations of the refractive indices with internal different components make biotissues become a high scattering medium for visible and near-infrared wavelengths, i.e., the therapeutic and diagnostic optical window.[1,2,3,4,5] Multiple scattering and absorption attenuate the effective light intensity of reaching internal tissue and diminish the detecting depth. They limit the clinical application of optical imaging techniques. It has the significant potential to improve the application of spectroscopic and optical imaging techniques in clinic
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