Abstract
A dual-wavelength digital holographic microscopy with premagnification is proposed to obtain the object surface measurements over the large gradient. The quantitative phase images of specimens are captured in high precision by the processing of filtering and phase compensation. The phase images are acquired without phase unwrapping, which is necessary in traditional digital holographic microscopy; thereby the proposed system can greatly increase the speed of reconstruction. The results of numerical simulation and optical experiments demonstrated that the reconstructed speed increased by 37.9 times, and the relative error of measurement is 4% compared with the traditional holographic microscopy system. It means that the proposed system can directly acquire the higher quality quantitative phase distribution for specimens.
Highlights
Digital holographic microscopy (DHM) is a powerful technology for the measurement of microscopic samples by recording and reconstructing the amplitude and phase of the wave
The dual-wavelength digital holographic microscopy uses two different wavelength lasers to simultaneously record the hologram and numerically reconstruct the phase information according to phase distribution under two wavelengths
The dualwavelength phase-shifting digital holography that selectively extracts wavelength information from five wavelength-multiplexed holograms is presented by Tahara, Tatsuki, et al [4]
Summary
Digital holographic microscopy (DHM) is a powerful technology for the measurement of microscopic samples by recording and reconstructing the amplitude and phase of the wave. A solution for absolute phase measurements was presented by Cuche Etienne et al [2] They introduced digital reference wave and phase mask, which were applied in phase-contrast imaging and optical meteorology. If the optical path difference is less than the equivalent wavelength, the real phase distribution of object can be directly obtained without phase unwrapping by the dualwavelength digital holographic interferometry. A phase-imaging technique to quantitatively study the three-dimensional structure of cells by simultaneous dual-wavelength reflection digital holography was presented by A. The principle of surface topography measurement and phase unwrapping method based on dual wavelength digital holography is introduced. Compared with the traditional single wavelength DHM, the system can obtain the phase information without phase unwrapping, and get the low noise and high precision quantitative phase images
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
