Abstract
BackgroundIntramuscular fat (intramuscular fat, IMF) is one of the important traits of pork quality. How to reasonably improve the intramuscular fat content is the most focus researchers. Some possible regulation of intramuscular fat deposition of candidate genes to cause the attention of people. The objective of this study was to elucidate the relationship between peroxisome proliferator-activated receptor γ (PPARγ) and adipose differentiation-related protein (ADRP) mRNA expression and intramuscular fat (IMF) deposition in the muscle tissue of three breeds of pig: Laiwu (LW), Lulai Black (LL), and Large White (LY).Results qPCR analysis of the PPARγ and ADRP genes in the three breeds of pig revealed PPARγ and ADRP mRNA expression profiles of LW > LL > LY and LL > LW > LY, respectively. PPARγ mRNA expression was significantly and positively correlated with IMF deposition (p < 0.05). There were significant correlations between PPARγ and ADRP mRNA expression levels (p < 0.01).ConclusionsThese results suggest correlations between PPARγ and ADRP in fat deposition and regulation in pigs, PPARγ gene may be a main effector of IMF content and play an important role during adipocyte differentiation in pigs, thereby providing new information to further elucidate molecular mechanisms associated with intramuscular fat deposition in Laiwu pigs and provides new data for further molecular studies of mechanisms underlying intramuscular fat deposition in human obesity. The continued elucidation of specific genetic mechanisms between PPARγ and ADRP warrants further studies.
Highlights
IntroductionIntramuscular fat (intramuscular fat, IMF) is one of the important traits of pork quality
Intramuscular fat is one of the important traits of pork quality
peroxisome proliferator-activated receptor γ (PPARγ) and adipose differentiation-related protein (ADRP) mRNA expression level As shown in Fig. 1, there were significant differences in PPARγ mRNA expression levels among breeds (p < 0.05), with highest values in LW followed by Lulai Black (LL) and Large White (LY)
Summary
Intramuscular fat (intramuscular fat, IMF) is one of the important traits of pork quality. The objective of this study was to elucidate the relationship between peroxisome proliferator-activated receptor γ (PPARγ) and adipose differentiation-related protein (ADRP) mRNA expression and intramuscular fat (IMF) deposition in the muscle tissue of three breeds of pig: Laiwu (LW), Lulai Black (LL), and Large White (LY). The search for candidate genes and molecular mechanisms associated with IMF deposition has become an important strategy in IMF research. Cui et al SpringerPlus (2016) 5:1501 mechanisms associated with the regulation of porcine intramuscular fat deposition, with the goal to reasonably improve IMF content. Previous research has shown that PPARγ, which is predominantly expressed in adipose tissue and the liver, is a master regulator of adipogenesis and controls adipocyte differentiation, proliferation, and lipid accumulation (He et al 2013). PPARγ activity can be adjusted by various methods and materials to increase PGRN polypeptide levels (Yang et al 2013)
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