Study on Quality Standard of Processed Curcuma Longa Radix.

Zhimin Chen,Haiting Zhou,Yongfeng Zhao,Zhuo Yang,Changjiang Hu,Liang Quan,Dong Cao,Wenbing Li

doi:10.1155/2017/2830754

Abstract

To control the quality of Curcuma Longa Radix by establishing quality standards, this paper increased the contents of extract and volatile oil determination. Meanwhile, the curcumin was selected as the internal marker, and the relative correlation factors (RCFs) of demethoxycurcumin and bisdemethoxycurcumin were established by high performance liquid chromatography (HPLC). The contents of multicomponents were calculated based on their RCFs. The rationality and feasibility of the methods were evaluated by comparison of the quantitative results between external standard method (ESM) and quantitative analysis of multicomponents by single-marker (QAMS). Ethanol extracts ranged from 9.749 to 15.644% and the mean value was 13.473%. The volatile oil ranged from 0.45 to 0.90 mL/100 g and the mean value was 0.66 mL/100 g. This method was accurate and feasible and could provide a reference for further comprehensive and effective control of the quality standard of Curcuma Longa Radix and its processed products.

Highlights

Curcuma Radix (Yujin) is derived from the dried root of Curcuma wenyujin, Curcuma longa, Curcuma kwangsiensis, or Curcuma phaeocaulis, which first appeared in the Theory of Drug Properties (Yao Xinglun) and has the effects of invigorating the circulation of blood, relieving pain, dispersing stagnated qi for relieving qi stagnation, clearing heat of heart and cooling blood, and curing jaundice [1,2,3]
The negative control sample had no peaks at the corresponding positions of Curcuma Longa Radix sample, which illustrated the other medicine herbs did not interfere with determination
Comparing results of quantitative analysis of multicomponents by single-marker (QAMS) and external standard method (ESM), we find that the relative standard deviation (RSD) were within 3%

Summary

Introduction

Curcuma Radix (Yujin) is derived from the dried root of Curcuma wenyujin, Curcuma longa, Curcuma kwangsiensis, or Curcuma phaeocaulis, which first appeared in the Theory of Drug Properties (Yao Xinglun) and has the effects of invigorating the circulation of blood, relieving pain, dispersing stagnated qi for relieving qi stagnation, clearing heat of heart and cooling blood, and curing jaundice [1,2,3]. In the Chinese Pharmacopoeia 2015 edition, only moisture and total ash are used as indicators for quality control of Curcuma Radix. It is difficult to fully reflect and control the quality of Curcuma Longa Radix due to the lack of quality control indicators. HPLC has applied in determination of curcuminoids in Curcuma Longa Radix simultaneously [14]. In order to explore a comprehensive quality control criterion for Curcuma Longa Radix and guarantee the effective and safe use of clinical practice. We added the contents of extract and volatile oil determination and developed and validated a quantitative analysis of multicomponents by single-marker (QAMS) for the simultaneous determination of polar active components in Curcuma Longa Radix, including curcumin, demethoxycurcumin, and bisdemethoxycurcumin

Methods

Results

Conclusion