Abstract

目的探讨T细胞疫苗(TCV) 延长异种移植胎肠存活时间的作用及其机制.方法将大鼠→小鼠游离胎肠移植模型分为供体特异性TCV(WTCV)免疫组、无关异种抗原TCV(LTCV)免疫组和单纯移植组.观察移植物各时相点的存活率,并用原位杂交法检测移植物内细胞因子mRNA表达,及用四甲基偶氮唑蓝(MTT)法测定单向混合淋巴细胞反应.结果 WTCV和LTCV免疫组移植物各时相点的存活率均显著高于单纯移植组,WTCV免疫组又高于LTCV免疫组(P<0.01).WTCV组对混合淋巴细胞反应(MLR)的抑制率高于LTCV组(P<0.01).WTCV免疫后,异种移植物内各细胞因子mRNA的表达均显著下调,其中以白介素-4(IL-4)mRNA表达下降最为显著(P<0.01).结论 (1)供体特异性TCV能延长异种移植物存活时间,其作用具有抗原特异和非特异的双重性;(2)WTCV延长异种移植物存活时间的作用可能与诱导移植物免疫耐受以及移植物内Th2细胞功能下调有关.

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