Abstract
Biosensors of single-gene knockout mutants and physical methods using mesh and quartz glass are employed to discriminate plasma agents and assess their lethal effects generated in a Direct-Current atmospheric-pressure oxygen plasma jet. Radicals generated in plasma are determined by optical emission spectroscopy, along with the O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> density measurement by UV absorption spectroscopy. Besides, thermal effect is investigated by an infrared camera. The biosensors include three kinds of Escherichia coli (E. coli) K-12 substrains with their mutants, totalling 8 kinds of bacteria. Results show that oxidative stress plays a main role in the inactivation process. Rather than superoxide O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-</sup> , neutral reactive oxygen species such as O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> and O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> ( <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">a</i> <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sup> <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Δ</i> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">g</sub> ) are identified as dominant sources for oxidative stress. In addition, DNA damage caused by oxidation is found to be an important destruction mechanism.
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