Abstract
Nitrogen-containing plasma polymerization is of considerable interest for tissue engineering due to their properties on cell adhesion and mesenchymal stem cells (MSCs) response. In this study, low-pressure RF plasma of acetylene and nitrogen was used to deposit nitrogen-containing plasma polymerized coatings on several substrates. Deposition kinetics and surface characteristics of coatings were investigated in terms of RF power and gas flow ratio. OES was used to monitor the plasma process and investigate the relation between the film structure and plasma species. Presence of several bonds and low concentration of amine functional groups were determined using FTIR and Colorimetric methods. Contact angle goniometry results indicated about 30% increase in surface hydrophilicity. Stability of coatings in air and two different liquid environments was examined by repeating surface free energy measurements. Deposited films exhibited acceptable stability during the storage duration. Surface roughness measured by AFM was found to decrease with growing concentration of nitrogen. The deposition rate increased with increasing RF power and decreased with growing concentration of nitrogen. Zeta potential measurements of coatings revealed the negative potential on the surface of the thin films. Temporary suppression of collagen X in the presence of plasma coatings was confirmed by RT-PCR results.
Highlights
The use of plasma technology in the context of plasma medicine for wound healing, sterilization of various surfaces, inactivation of pathogens in contact with living tissues, selective treatment of cancer cells, and for dentistry and dermatological applications is a growing important research area[1,2,3,4,5]
One of distinct problems with current cartilage repair techniques is that mesenchymal stem cells (MSCs) from osteoarthritis (OA) patients rapidly express type X collagen (COLX), a marker of late stage chondrocyte hypertrophy associated with endochondral ossification; they will not be able to be used effectively in treatments as a source of autologous stem cells
The active species generated during the discharge were detected with optical emission spectroscopy (OES)
Summary
Received: 6 October 2016 Accepted: 21 July 2017 Published: xx xx xxxx polymerization in C2H2/N2 plasma and Their Impact on COL X. In 2005, Nelea et al.[24] investigated the effect of thin plasma-polymer films, known as nitrogen rich plasma-polymerized ethylene (PPE:N), on the behavior of COL X In a period of 14 days They used ethylene and ammonia gases to deposit the films by an RF-PECVD coating process. In 2011, Rampersad et al.[25] conducted a study to determine if the suppression of COL X by PPE:N is maintained when MSCs are transferred to pellet cultures in serum-free media They used both low- and high-pressure plasma systems for synthesis of PPE:N films. Physio-chemical properties of the thin films including roughness, hydrophobicity, zeta potential, surface charge, and active functional groups govern factors in the biological environments[26] It was not well understood which mechanisms control MSCs differentiation[23]. The response of MSCs and expression of COL X, under the influence of plasma coatings, were studied
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