Abstract
Summary The intracellular parasite Neospora caninum is prevalent in several countries and is increasin gly recognized as an important cause of abortion and st illbirth in cattle. For characterizeing the tachyzo ite antigens of Neospora caninum in aborted cows, sera were obtained from 116 cows which were aborted in the third semester of the pregnancy period and had anti bodies to Neospora caninum in ELISA. To obtain the protein content of Neospora , purified tachyzoites were lysed, electrophoretica lly separated and blotted to nitrocelloluse membrane for immunostaining. Minimum 9 and maximum 13 protein bands ranging from 10 to 90 kDa were observed after immunostaining. It se ems that, in almost all of the cows, two protein ba nds with a molecular weight of 45 and 41 kDa, have a pr ominent reaction in Western blotting. According to our findings, these two protein bands are the most impo rtant antigens observed after Western blotting, in seropositive aborted cows.
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