Study on nitrogen removal performance and microbial community structure succession under the condition of anaerobic sludge and low carbon source

Jiabin Wang,Cong Yu,Kang Xie,Tianhang Li,Xiaoyu Wang,Zeya Wang,C.-H Weng,R Weerasinghe

doi:10.1051/e3sconf/20185303049

Abstract

Under anaerobic low carbon source, the reactor was started-up by inoculating anaerobic sludge. The nitrogen removal efficiency and nitrogen conversion pathway during the start-up process were analyzed. At the same time, the structure succession of bacteria in the process of start-up was analyzed by PCR-DGGE technology from molecular biology level. The results showed that inoculated anaerobic sludge had a good effect on nitrogen removal under the condition of anaerobic low carbon source.

Highlights

In recent years, a large amount of nitrogen containing substances produced in the process of production and living have entered the water, causing serious pollution to the water environment due to the development of industry and agriculture and the improvement of the people's living standard[1,2]
Anaerobic sludge was inoculated in a ASBR reactor, and its nitrogen removal characteristics and microbial community structure succession were studied under anaerobic and low carbon source conditions
2.3.2 Molecular biological analysis method In order to clarify the principle of Microbiology in the process of start-up systematically, the microbial population structure and succession law of the sludge were carried out by PCR-DGGE technology for 2 months and 4 months

Summary

Introduction

A large amount of nitrogen containing substances produced in the process of production and living have entered the water , causing serious pollution to the water environment due to the development of industry and agriculture and the improvement of the people's living standard[1,2]. Nitrification requires adequate dissolved oxygen, and denitrification requires carbon source , which increased the cost of the wastewater denitrification process[5,6,7]. It has attracted wide attention from researchers that how to remove nitrogen economically and efficiently. Anaerobic sludge was inoculated in a ASBR reactor, and its nitrogen removal characteristics and microbial community structure succession were studied under anaerobic and low carbon source conditions. It is 700mm high, and the inner diameter is 80mm,which has the air outlet, the air intake, the water outlet, the water intake. The reactor was stirred by nitrogen circulation and stirred 15min per hour.

Experimental method

Findings

Analysis and discussion of the results