Abstract
目的 探讨烟煤尘、无烟煤尘及褐煤尘的致突变作用.方法 分别用烟煤、无烟煤和褐煤粉尘的二甲基亚砜混悬液,作鼠伤寒沙门氏菌回复突变(Ames)和姐妹染色单体交换(SCE)试验.Ames试验采用TA97、TA98、TA100和TA102菌株,并将3种煤尘各分为5000、500、50和5 μg/皿4个剂量组;SCE试验采用健康成人外周血淋巴细胞,煤尘样品分为500、50和5 μg/皿3个剂量组.各剂量组均设3个平行样.试验分为加与不加体外代谢活化系统(S9)两部分,同时设阴性和阳性对照组.Ames试验在细菌培养(37℃)48 h后计算回变菌落数;SCE试验在标本37℃培养72 h后制成染色体标本玻片,油镜下观察计数细胞第二增殖周期分裂相的SCE频率.结果 3种煤尘在加S9与不加S9时,其菌落数均不超过自发回变菌落数的2倍,而且均不能使SCE频率明显升高.结论 在本实验条件下,烟煤尘、无烟煤尘及褐煤尘均未见致突变作用。
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Chinese Journal of Industrial Hygiene and Occupational Diseases
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
