Abstract
Staphylococcus aureus remains a major health problem responsible for many epidemic outbreaks. Therefore, the development of efficient and rapid methods for studying molecular profiles of S. aureus strains for its further typing is in high demand. Among many techniques, matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI TOF MS) represents a timely, cost-effective, and reliable strain typing approach, which is still rarely used due to insufficient knowledge about the impact of sample preparation and analysis conditions on the molecular profiles and strain classification efficiency of S. aureus. The aim of this study was to evaluate the effect of the culture conditions and matrix type on the differentiation of molecular profiles of various S. aureus strains via the MALDI TOF MS analysis and different computational methods. The analysis revealed that by changing the culture conditions, matrix type, as well as a statistical method, the differentiation of S. aureus strains can be significantly improved. Therefore, to accelerate the incorporation of the MALDI-based strain typing in routine laboratories, further studies on the standardization and searching of optimal conditions on a larger number of isolates and bacterial species are of great need.
Highlights
Staphylococcus aureus is a Gram-positive bacterium, the habitats of which are the nasal membranes and skin of warm-blooded animals
The analysis of the score values revealed that the impact of the culture medium composition is highly dependent on the matrix used as well as the type of MS data: raw spectra or Main Spectra (MSP) (Table 1)
In the HCCA variants, bacteria cultured on two media, blood agar (BLA) and CHROMagarTM Orientation (CHRA), were characterized by the highest score values
Summary
Staphylococcus aureus is a Gram-positive bacterium, the habitats of which are the nasal membranes and skin of warm-blooded animals. Due to secretion of extracellular factors and toxins as well as invasive properties, such as adherence, biofilm formation, and resistance to phagocytosis, it is the causative agent of many life-threatening invasive diseases [1]. S. aureus remains a major health problem around the world since it is considered a leading cause of a wide range of hospitaland community-acquired infections [2,3,4,5,6]. S. aureus demonstrates a high capability to adapt to a variety of conditions; its clones may spread very . Important is rapid discrimination between methicillin-resistant (MRSA)
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