Study on mitochondrial ultrastructure, trace elements and their correlative factors in cells of gastric mucosa of the gastropathic patients with Spleen deficiency syndrome

Abstract

After examining 88 gastropathic patients with Spleen deficiency syndrome by using transmission electron microscope, X-ray energy disperse analysis system, histochemical staining and radioimmuno methods, the authors found that the gastric mucosa cyclic adenosine monophosphate, superoxide dismutase level, quantity of mitochondria and its crista, the ratio of diameter between ventricle and cavity of mitochondria and the content of zinc (Zn), copper (Cu) of mitochondria were decreasing to certain extent which tends to get lower and lower with different groups in the order of health control group; Spleen Qi deficiency group and Spleen deficiency with Qi stagnation group; chronic superficial gastritis group, chronic atrophic gastritis group and gastric cancer group; complete small intestinal metaplasia (IM) group, incomplete small IM group, complete colonic IM group and incomplete colonic IM group (P<0.05~0.001). While the degeneration rate of mitochondria, Cu/Zn ratio, metaplasia rate of gastric mucosa, rate of incomplete colonic IM and content of lipid peroxide were increasing in the above order (P<0.05~0.001). It is suggested that the comprehensive effect of the degeneration of mitochondria and the quantitative changes of its correlative factors is the physiopathologic base for inducing Spleen deficiency disease, gastric mucosa IM and canceration.