Abstract
A novel and highly effective Acinetobacter (L15) were obtained from soil by morphological observation, physiological and biochemical tests and 16 SrDNA sequencing. The optimal reaction environment of L15 at different temperatures was determined by shaking test, and the catalytic activity was measured. It was found that the bacteria could withstand n-cetane value above 5000 mg/L when the initial culture pH was 7, the inoculation amount was 5%, the shaking speed was 150 r/min, and the culture temperature was 30℃. Under the optimal 7-day fermentation condition, the degradation rate of n-cetane compounds reached 94.09%, and the decomposition rate of 3000 mg/L diesel oil was basically complete. The results showed that the bacteria had good potential for bioreactor in diesel contaminated soil.
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