Study on Mechanism of Shenqi Dihuang Decoction in Treating Diabetic Nephropathy Based on Network Pharmacology

Abstract

Objective: To explore the mechanism of Shenqi Dihuang Decoction in treating diabetic nephropathy based on network pharmacology. Methods: The component targets of Shenqi Dihuang Decoction and diabetic nephropathy (DKD) targets were searched and screened by network database. Using Cytoscape3.7.2 software to construct a network of traditional Chinese medicine components-targets; The protein-protein interaction network (PPI) of Shenqi Dihuang Decoction for CRF treatment was constructed using STRING database, and the core targets were found out through topological analysis. Finally, biological pathway and enrichment analysis were carried out on drug-disease intersection targets. Results: A total of 106 active ingredients of Shenqi Dihuang Decoction were collected. 1532 corresponding targets; DKD disease targets 533; There are 48 common targets for drug components and diseases. Topological analysis was performed with a Degree value 2 times (14.3) higher than the average, and 4 key core targets were finally obtained. Biological process and enrichment analysis show that the prescription mainly involves 13 biological processes, 4 KEGG pathways and 3 typical pathways in treating DKD, mainly involving cell response to hormone stimulation, followed by biological processes such as neuroactive ligand-receptor interaction KEGG pathway, blood circulation, cell response to nitrogen compounds, chemical synaptic transmission, etc. Conclusion: Shenqi Dihuang Decoction mainly acts on PTGS2, NR3C1, NOS3, ESR1 and other targets. Its targets are many and its mechanism is complex. It may play a role mainly through the biological process of regulating the response degree of cells to hormone stimulation.