Study on Measuring Proteolytic Activity and Phyto Chemical Analysis of Fruit Latex and Leaves Extract of Papaya (Carica papaya L.) Cv. CO2

Abstract

Background: Papaya latex is a milky fluid secreted by ducts and is a complex mixture of phytochemicals (secondary metabolites). Lowry’s method was used to quantify the papain extracts from the fruits and leaves to measure cysteine protease activity. Methods: The experiment was conducted using the extracts of papaya fruit latex (Papain) and leaf extracts for phytochemical analysis. As a result, the enzyme concentration in crude extracts was highest in the fruit latex and lowest in the leaves. The dialyzed leaves extract band/color was very light in comparison to the fruit latex band/color, indicating that the enzyme concentration in the crude leaves sample was the lowest. Result: Quantitative estimation of protein content was observed higher in papaya fruit latex papain (82.5 mg/ml) and in papaya leaves (36.6 mg/ml). Positively higher significant results were also confirmed for the protease activity examined for latex and leaves valued 6.34 mg and 5.323 mg, respectively, whereas, the similar results were noted for the content of phytochemical constituents in fruit latex for glycosides, saponins and terpenoids as well as in leave extract for glycosides, flavonoid, phenol, reducing sugar, tannins, terpenoids, saponins.