Study on Laccase Immobilization with PVA Materials

Abstract

This article used glutaraldehyde to immobilize laccase and then used the immobilization laccase to treatment papermaking effluent. The study first discussed the effect of PVA crosslinking degree, epoxidation temperature and epoxidation time for the performance of PVA carrier. The research found 100g PVA ( mass fraction is 10%), 35mL glutaraldehyde, 10mL DMSO and 10mL epoxy chloropropane reacted 6h in 60 °C, pH=11-12 and surface amount of epoxidation reached the max value. its amount is 0.3073mmol . And then the laccase were fixed with the max value. The optimum conditions of immobilization laccase treated papermaking effluent were researched. The effects of treated time, temperature, pH value, the dosage of enzyme and HBT, the removal value of CODCr and decolorization were tested. The effects of treated time, temperature, pH value, the dosage of enzyme and HBT, concentration of Cu2+ on the removal value of CODCr and decolorization were tested. It was found that the optimum conditions for immobilization laccase treatment were Cu2+ 0.006μmol/L, HBT 0.008g/L, pH=4, temperature 50°C, reation time 6h (with air injected). The result showed that the CODCr removal rate reached 63.15%, the decolorization rate reached 61.53% in 8h. Furthermore BOD/COD of chemical pulp effluent was increased from 0.17 to 0.455. Comparing with dissociation laccase, stabilities of immobilization laccase for hot and pH increased. Through this research a new advanced treatment method can be found. This method can improve biodegradation performance of papermaking effluent. Papermaking effluent treated by aerobic, anaerobic and laccase system will achieve standard discharge.