Study on Intervention Mechanism of Yiqi Huayu Jiedu Decoction on ARDS Based on Network Pharmacology.

Changyong Luo,Yan Li,Xin Li,Xu Liang,Qingqiao Sun,Caihua Xiong,Yanpeng Zeng,Shujing Zhang,Yuanhong Ma,Qian Wang

doi:10.1155/2020/4782470

Abstract

Background Yiqi Huayu Jiedu (YQHYJD) is a traditional Chinese medicine decoction made up of eight traditional Chinese medicines. Although YQHYJD is effectively used to prevent and treat ARDS/acute lung injury (ALI) in rats, the molecular mechanisms supporting its clinical application remain elusive. The purpose of the current study was to understand its lung protective effects at the molecular level using network pharmacology approach. Methods In an ARDS animal model, the beneficial pharmacological activities of YQHYJD were confirmed by reduced lung tissue damage levels observed on drug treated rats versus control group. We then proposed a network analysis to discover the key nodes based on drugs and disease network. Subsequently, we analyzed interaction networks and screened key targets. Using Western blot to detect the expression level of key targets, the intervention effect of changes in expression level of key targets on ARDS was evaluated. Results Pathway enrichment analysis of highly ranked genes showed that ErbB pathways were highly related to ARDS. Finally, western blot results showed decreased level of the AKT1 and KRAS/NRAS/HRAS protein in the lung after treatment which confirmed the hypothesis. Conclusion In conclusion, our results suggest that YQHYJD can exert lung tissue protective effect against the severe injury through multiple pathways, including the endothelial cells permeability improvement, inflammatory reaction inhibition, edema, and lung tissue hemorrhage reduction.

Highlights

Yiqi Huayu Jiedu (YQHYJD) is a traditional Chinese medicine decoction made up of eight traditional Chinese medicines
In the previous meta-analysis study, we found that the combination of traditional Chinese and Western medicine in the treatment of Acute respiratory distress syndrome (ARDS) has significant advantages in improving the mortality rate and reducing the time of mechanical ventilation compared with simple support treatment [13]
According to the clinical observation for many years, we found that the series prescription of YQHYJD had a significant effect on the treatment of critical respiratory system diseases [29], and our previous work [30,31,32,33] shows that YQHYJD has protective effects on lung injury caused by endotoxin in ARDS rats. e compound can reduce alveolar collapse, reduce inflammatory cell exudation, and at the same time reduce pulmonary edema and improve lung permeability

Summary

Introduction

Yiqi Huayu Jiedu (YQHYJD) is a traditional Chinese medicine decoction made up of eight traditional Chinese medicines. YQHYJD is effectively used to prevent and treat ARDS/acute lung injury (ALI) in rats, the molecular mechanisms supporting its clinical application remain elusive. E purpose of the current study was to understand its lung protective effects at the molecular level using network pharmacology approach. In an ARDS animal model, the beneficial pharmacological activities of YQHYJD were confirmed by reduced lung tissue damage levels observed on drug treated rats versus control group. Our results suggest that YQHYJD can exert lung tissue protective effect against the severe injury through multiple pathways, including the endothelial cells permeability improvement, inflammatory reaction inhibition, edema, and lung tissue hemorrhage reduction. Related studies have shown that TCM has obvious effects in reducing ARDS/ALI lung tissue inflammation, reducing mortality, and improving prognosis. Compared with a single blocker, it has better clinical advantages in treating diseases, and has broad application prospects for the prevention and treatment of ARDS [7,8,9]

Methods

Results

Discussion

Conclusion