Abstract
The effects of streptomycin sulfate, carbendazim, penicillin and nystatin on the inhibition of fungi and bacteria in blueberry explants were studied. The results showed that the addition of streptomycin sulfate or penicillin with different concentrations in the medium had a certain inhibitory and killing effect on bacteria in blueberry explant contamination, while the addition of carbendazim or nystatin had a certain inhibitory and killing effect on fungi in blueberry explant contamination. When the concentration of streptomycin sulfate in the medium reached 60units / mL, the highest survival rate was 44.0%, and the bacterial infection rate was the lowest. When the concentration of carbendazim in the medium was 0.4 mg/mL, the survival rate reached 40.5%, and the fungal infection rate decreased greatly. When the penicillin concentration in the medium reached 40mg/mL, the lowest infection rate of bacteria was 4.0%, and the highest survival rate was 31.5%. When the concentration of nystatin in the medium reached 40mg/L, the highest survival rate was 34.5%. Therefore, streptomycin sulfate had better inhibitory effect on bacteria, while carbendazim had better inhibitory effect on fungi, which provided reference for future research.
Highlights
Plant tissue culture technology has very strong practicability in contemporary society
With the increase of streptomycin sulfate concentration, the bacterial infection rate decreased to 13%, and the range was large
With the increase of concentration, the bacterial infection rate even increased from 17.5% to 30.0%, indicating that carbendazim could effectively act on fungi and had no effect on bacteria
Summary
Plant tissue culture technology has very strong practicability in contemporary society. It is used in different fields and has very strong application value in scientific experiments and production[1]. The widespread existence of microorganisms has become a major obstacle to the development of tissue culture technology. In the process of explant culture, it is easy to be contaminated by bacteria or fungi in the environment, affecting the survival rate of tissue culture, which requires us to find appropriate antibacterial methods in the experimental process. Antibiotics have significant inhibitory effects on bacteria and fungi in plant tissue culture. Antibiotics are bioactive substances produced by some organisms, which are resistant to pathogens or have other effects, and can inhibit the development and metabolism of other microorganisms[2]. With the continuous progress of society, antibiotics are more widely used in the prevention and treatment of explants pollution
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