Study on in-vivo anti-tumor activity of Verbena officinalis extract.

Ying Wang,Jing Liu,Jun Yang,Su-Ling Xu,Zhi-Fen Wang,Qing-Hui Yang,Wei-Zheng Kou

doi:10.4314/ajtcam.v10i3.19

Abstract

We investigated the anti-tumor effects of Verbena officinalis extract on H22 tumor-bearing mice and its effect on immune function. Mice model of H22 solid tumor was established, the mice were divided into five groups and administered the extract, later, tumors were removed and inhibition rates were calculated; spleens were removed and spleen indices were calculated, and the sheep red blood cell-delayed-type hypersensitivity (SRBC-DTH) and the serum hemolysin level were determined. The Verbena officinalis extract had anti-tumor effect, with the inhibition rate reaching 38.78%, it also increased the spleen index to a certain extent, in addition, the changes in DTA and HA were not obvious compared with the model group. The Verbena officinalis extract had in vivo anti-tumor effect, while causing no damage on the immune function.

Highlights

Verbena officinalis is the dried aerial parts of Verbena officinalis L. and bought from Zaoyang City in Hubei province, which belongs to the Verbenaceae family (Chinese Pharmacopoeia Commission, 2010), it is mainly used for the treatment of abdominal mass, amenorrhea, dysmenorrhea, malaria, pharyngitis, carbuncles, edema, etc
N et al, 1998)(not in the references list), antitussive (Gui et al, 1985) effects as well as the effect on uterine smooth muscle (Reproductive Physiology Group i et al, 1974), recent studies have reported the inhibitory effect of Verbena officinalis extract on human choriocarcinoma JAR cells and its mechanism of action has been studied (Xu et al, 2001; Zhang et al, 2005; Wang et al, 2004)
The results for the effect of Verbena officinalis extract on tumor weight and tumor inhibition rate were as shown in Table 2, tumor weights between the model group and high-dose Verbena officinalis group had a very significant difference (P

Summary

Introduction

Verbena officinalis is the dried aerial parts of Verbena officinalis L. and bought from Zaoyang City in Hubei province, which belongs to the Verbenaceae family (Chinese Pharmacopoeia Commission, 2010), it is mainly used for the treatment of abdominal mass, amenorrhea, dysmenorrhea, malaria, pharyngitis, carbuncles, edema, etc. The main chemical constituents of Verbena officinalis include iridoid glycosides such as verbenalin and its derivatives (Tian et al, 2005; Zhang et al, 2000), and flavonoid compounds such as luteolin, kaempferol (Chen et al, 2006), ursolic acid (Ma et al, 2005), and volatile oils (Khaled et al, 2010). Studies on its pharmacological activities have mainly focused on the anti-inflammatory (Calvo et al, 2006; Vilalta et al, 1998; M.I. Calvo, N et al, 1998)(not in the references list), antitussive (Gui et al, 1985) effects as well as the effect on uterine smooth muscle (Reproductive Physiology Group i et al, 1974), recent studies have reported the inhibitory effect of Verbena officinalis extract on human choriocarcinoma JAR cells and its mechanism of action has been studied (Xu et al, 2001; Zhang et al, 2005; Wang et al, 2004). The anti-tumor effect of Verbena officinalis extract was studied with the mice H22 hepatoma ascites as the animal model, and its anti-tumor mechanism was initially investigated

Methods

Results

Conclusion