Study on IFN-γ/TGF-β level in a model of acute inflammation and melanoma

Abstract

Objective:To build a mouse tumor model with a manufactured surgical wound representing acute inflammation,and to evaluate the relationship between acute inflammation or wound healing and the process of tumor growth.Then to observe the impact of IFN-γ/TGF-β on tumor growth.Methods:Male C57BL mice of six weeks were used and divided into the experiment group and the control group.The B16F10 melanoma cell suspension was injected into the left groin area of each mouse.A wound measured 1 cm in diameter was built on the opposite side of bodies in the experiment group when tumor volume was about 0.5 cm~3. The expression of IFN-γ/TGF-β in blood serum and tumor tissues were examined by ELISA.In order to further confirm the effect of TGF-β on tumor growth,another 16 mice models with melanoma were established and 8 of them received IFN-γ injection (the experiment group).Results:When acute inflammation had influences on tumor,a two-phase development was presented.In the early phase,the growth of tumor in the mice with wound was slower than that in the control group.In the early phase,the release of IFN-γ was higher and the release of TGF-β was lower in the experiment group.In the later phase,the growth of tumor in the mice with wound was similar to that in the controls and the release of TGF-β was higher.In vivo experiment confirmed the above results.In the early phase,the release of TGF-β was not significantly different between the experiment group and the control group (P0.05).In the later phase,the release of TGF-β in the experiment group was higher than that in the control group (P0.05).Conclusion:In the early phase of acute inflamma- tion,inhibitory effects of IFN-γ on tumor growth were presented.In the later phase,the inhibited tumor was resistant to IFN-γ through the release of TGF-β to balance the effect of inflammatory factors on tumor cells.