Study on germination of sunflower seeds that affected by ROS.

Abstract

This study investigated in order to evaluation of relationship between germination and produce of reactive oxygen species in sunflower seeds. This study was performed in two separate experiments, each in a completely randomized design with factorial design with four replications. In both experiments, uses from dormant and non dormant seeds of sunflower. It also applies of treatments Methylviologen and Cyanide in dormant seeds which are the producers of reactive oxygen species. Finally, germination, the content of reactive oxygen species such as hydrogen peroxide and superoxide anion production were evaluated as well. The results showed that the main reason for release of sunflower seeds dormancy is production of reactive oxygen species is an acceptable level so that seed germination of dormant seeds which was treated with Methylviologen and Cyanide was more than dormant control seeds and was similar to non dormant seeds. Also, production of Hydrogen peroxide and superoxide anion production of seeds treated with Methylviologen and Cyanide was similar to non dormant seeds and they had nearly doubled of dormant seeds.