Abstract
In this study, near-infrared (NIR) Raman spectroscopy was explored to assess the incorporation of calcium hydroxyapatite (CHA~960cm−1) and other biochemical substances during the recovery of rabbits with complete radial fractures treated with or without small splints. 24 rabbits were randomy divided into two groups, one treated with small-splint-fixation therapy and the other without any intervention. The rabbits were sacrificed at 7, 15, 23, and 30 days after surgery, and the surface layers of the calluses in the fracture healing site from control and treated groups were routinely prepared for Raman spectroscopy. The prominent Raman bands were observed, including minerals at 430, 590, 960, 1003, and 1071 cm−1, protein at 856, 876, 1246, and 1667cm−1, and lipid at 1767cm−1. The carbonate-to-phosphate ratio (CO3toυ1PO4) and the mineral-to-matrix ratio (υ1PO4to amide I) were calculated from these normalized Raman bands. Comparison of theυ1PO4-to-amide I ratio for the control group with that of the treated group probably indicated that the small-splint-fixation therapy could be useful for the gradual mineralization of the collagen matrix during fracture healing.
Highlights
Bone is a special material integrating an organic matrix, principally type I collagen, and an inorganic component [1]
Important aspects of bone tissue quality include the physicochemical properties of its main constituents, the organic matrix, and the mineral crystals
Prominent Raman bands are observed in the following peak positions with tentative biomolecular assignments: 430 cm−1, 590 cm−1, 960 cm−1 (P–O symmetric stretch vibration of hydroxyapatite), 1003 cm−1, 1071 cm−1 (C–O in plane stretch vibration of carbonate hydroxyapatite), 1246 cm−1 (C–N–H stretch vibration of Amide III), 1450 cm−1 (C–H bending mode of protein and lipid), 1667 cm−1 (C–C–N stretch vibration of amide I), and so on
Summary
Bone is a special material integrating an organic matrix, principally type I collagen, and an inorganic component [1]. E xation therapy is the important and basic method to treat the bone fracture, and it is the fundamental condition that the bone fracture canbe concrescent on time [3]. As an important elastic xation therapy, small-splint- xation therapy ts on the trend of the healing of the fracture [6], whose guiding idea is “dynamic and static combination, equal stress on bones and muscles, exterior and interior treatment, and collaboration between doctors and patients.”. Microscopic examination and imaging are the cardinal methods for the assessment of small-splint xation therapy in promoting bone healing of rabbits with complete radial fractures nowadays. The in uence of small-splint- xation therapy on bone healing is still vague. The macroscopic properties of bone have been extensively examined and are somewhat established, the early steps in its mineralization are not well understood
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