Study on extraction technology of carrot extract containing panaxynol

Abstract

Objective To investigate and optimize the technology of extraction of panaxynol from carrot. MethodsUsing the concentration of panaxynol as indicator by high performance liquid chromatography(HPLC),the factors that affected the extraction of panaxynol,including extracting parts and method were investigated respectively by single factor method.Based on the preliminary result,the orthogonal test was designed to optimize the extraction technology.Flow cytometry was employed to observe the effect of the optimized extract and panaxynol on leukemia cell line HL60 in vitro. Results The press residue was determined as the raw material of extraction.The optimum conditions were that the ratio of material to liquid(petroleum ether) was 1∶1,times of ultrasonic extraction were three,and the duration of extraction was 2 h.Significant apoptosis of HL60 cells was revealed by flow cytometry after treatment of the extract(40 μg/mL) and panaxynol(5 μmol/L) for 12 h,respectively. Conclusion The optimized extraction method is simple,convenient and reasonably practicable,with which the efficiency of extraction of panaxynol from carrot is increased significantly,and the extract has significant effects on growth inhibition and apoptosis promotion of HL60 cells in vitro.