Study on expression and change of peripheral blood macrophsge migration inhibitory factor in patients with acute cerebral infarction

Abstract

[Objective]To explore the function of peripheral blood macrophage migration inhibitory factor (MIF) during cerebral infarction by studying the expression and change of MIF in patients with cerebral infarction.[Methods]The levels of plasma MIF were measured by enzyme linked immunosorbent assay (ELISA) and the levels of peripheral blood mononuclear cells '(PBMC) MIF mRNA were measured by real-time polymerase chain reaction (RT-PCR) in 68 patients at acute stage (1～3 days),subacute stage (10～14 days).Mean time,99 healthy persons mating cerebral infarction group in both sex and ages were measured.[Results]At acute stage,the levels of plasma M1F[(52.77 ± 25.42)ng/ml]and PBMC MIF mRNA (0.42 ± 0.37)in the patients with cerebral infarction were obviously higher than those of subacute stage[(39.20 ± 17.46)ng/ml,0.27 ± 0.29]and the healthy controls[(32.66 ± 27.92)ng/ml,0.24 ± 0.23](P ＜ 0.05).The levels of plasma MIF and PBMC MIF mRNA in the patients with cerebral infarction at subacute stage had no significant difference with those of the healthy controls (P＞ 0.05).[ConcIusions] The plasma level of MIF and the PBMC expression of MIF mRNA are in increased gradually,and then gradually drop down to normal level.Dynamic detections of the plasma level of MIF and the PBMC expression of MIF mRNA are useful for forecasting the development and prognosis of acute cerebral infarction. Key words: Cerebral infarction; Peripheral blood mononuclear cells; Macrophage migration inhibitory factor; Real-time polymerase chain reaction