Abstract
Objective: To investigate the protective effects and mechanisms of esculetin(Esc) on H9C2 cells injury induced by doxorubicin (DOX). Methods: H9C2 cells were cultured and divided into control group, model (DOX) group and intervention (ESC + DOX) group. Flow cytometry was used to detect apoptosis of H9C2 cells and reactive oxygen (ROS) level in H9C2 cells; Western blotting to detect the cleaved Caspase-3, cleaved PARP, bid and Bcl-2 protein expression in H9C2 cells. Results: The number of apoptosis and ROS level of H9C2 cells in the model group, the expression of cleaved Caspase-3, cleaved PARP and Bid protein in the model group were obviously higher than control group; and the Bcl-2 protein expression was obviously lower (P < 0.05).The number of apoptosis and ROS level, cleaved Caspase-3, cleaved PARP and Bid protein expression in H9C2 cells in intervention group were obviously lower than model group;the Bcl-2 protein expression was obviously higher (P< 0.05). Conclusion: Esculetin can reduce the cardiotoxicity induced by doxorubicin by reducing apoptosis and ROS level.
Highlights
Doxorubic in (DOX) is an anthracyc line antibiotic with strong anti-tumor effect,which is widely used as a potent antitumour drug, examples like breast cancer, lymphoma and melanoma
The cells in the control group were only added with culture medium, and the cells in the two Esc groups were added with 5μM, 10μM Esc culture for 2 hours; cells in each group were added with different concentrations of doxorubicin (0μM, 1μM, 2μM, 4μM, 8μM, 16μM) continue to culture for 48 hours
Apoptosis and reactive oxygen species (ROS) content of H9C2 cells: The apoptosis number and ROS content of H9C2 cells in the DOX group were obviously higher than control group, and the apoptosis number and ROS content of H9C2 cells in the Esc +DOX group were obviously lower than DOX group (P
Summary
Doxorubic in (DOX) is an anthracyc line antibiotic with strong anti-tumor effect,which is widely used as a potent antitumour drug, examples like breast cancer, lymphoma and melanoma. It can cause serious irreversible cardiomyopathy and congestive heart failure. Speaking, Esculetin is a hydroxycoumarin that is umbelliferone in which the hydrogen at position 6 is substituted by a hydroxy group. It is used in filters for absorption of ultraviolet light. A rat embryonic cardiomyocyte (H9C2 cells) injury model induced by doxorubicin was established to explore the possible mechanism of Esc in reducing the cardiotoxicity induced by doxorubicin
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