Abstract

Objective To study the effects of epithelial-mesenchymal transition(EMT) and transcription factors of Twist and Snail during the genesis and development of cervical squamous epithelial lesions. Methods From January 2005 to December 2009, a total of 170 cases of file paraffin tissue blocks of cervical squamous epithelial lesions in Jingzhou Maternal and Child Health Hospital were selected into this study. Among them, 20 cases of chronic cervicitis were included into chronic cervicitis group, 32 cases, 27 cases, 35 cases and 56 cases cervical intraepithelial neoplasia(CIN)Ⅰ, Ⅱ, Ⅲ and cervical squamous carcinoma were included into CINⅠgroup, CINⅡgroup, CINⅢ group and cervical squamous carcinoma group, respectively. Expression of Twist, Snail, E-cadherin and Vimentin in these samples were analyzed by in situ hybridization (ISH) and SABC immunohistochemical stain. Results With the process of chronic cervicitis to CIN to cervical squamous carcinoma, expression of E-cadherin was decreasing gradually, and the expression of Vimentin, Twist and Snail were increasing gradually. Expression difference of E-cadherin and Vimentin between the chronic cervicitis group and the cervical squamous carcinoma group (or CINⅢ group) were obvious (P<0.05, respectively). The positive rates of Twist and Snail in the cervical squamous carcinoma group were higher than those in other groups(P<0.05). The expression of Twist and Snail were negatively correlated with E-cadherin respectively(P<0.05), and Twist was positively correlated with Vimentin(P<0.05). Conclusions The down-regulation of E-cadherin and the up-regulation of Vimentin, Twist and Snail in cervix tissue, which may be important molecular biological signals in the genesis of cervical squamous carcinoma. Twist and Snail may promote the progression of cervical squamous epithelial lesions through mediating EMT, and lead to cervical squamous carcinoma eventually. Key words: epithelial-mesenchymal transition; Twist; Snail; cervical squamous epithelial lesion

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