Abstract
It has been considered as a crucial field for the extraction of active ingredients from herbal medicine to use a green and efficient method in the medicinal and food industries. In this study, a deep eutectic solvent (DES) coupled with microwave-assisted extraction (MAE) was used to extract ten compounds from Eucommia ulmoides leaves. Response surface methodology (RSM) combined with Box-Behnken design (BBD) was used to optimize the extraction conditions using the MAE method. An anti-solvent method efficiently recovered target analytes from the DES. By combining the DES-based MAE and quantitative analysis of multi-components by single mark (QAMS) methods, the contents of ten compounds in the leaves of Eucommia ulmoides were determined to clarify the relationship between the accumulation of secondary metabolites and the harvest period. This study provides a novel strategy for the harvesting, processing, and quality control of the raw materials from Eucommia ulmoides leaves.
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