Abstract
Objective To study the effect of vitrified-warmed juvenil mouse ovaries on the mRNA and protein expression of DNA methyltransferase 1 (Dnmt1) and growth factor receptor-binding protein 10 (Grb10). Methods Twenty-six 10-day-old C57BL/6 female mice were used in this experiment. The bilateral ovaries of each juvenile mouse were divided into two groups: the fresh group and the vitrified-warmed group. The expression of Dnmtl in follicles embedding was detected by immunohistochemistry. The expressions of Dnmt1 and Grb10 protein in fresh group and vitrified-warmed group were detected by Western blotting technology. The expressions of Dnmt1 and Grb10 mRNA in fresh group and vitrified-warmed group were detected by qRT-PCR. Results Dnmtl protein all appeared in the nucleus of granulosa cell and oocytes of fresh and vitrified-warmed juvenile mouse ovaries. The expressions of Dnmt1 protein and mRNA in vitrified-warmed juvenil mouse ovaries were remarkably decreased than that of fresh group (P<0.05). The expressions of Grb10 protein and mRNA in vitrified-warmed juvenil mouse ovaries were remarkably increased than that of fresh group (P<0.05). Conclusion Decreased expression of Dnmt1 in juvenil mouse ovaries induced by vitrified-warmed process may result in the overexpression of Grb10, which may lead to an increased risk of glucose metabolism epigenetic defects in the oogamete. Key words: Juvenil mouse ovaries; Vitrification; DNA methyltransferase 1 (Dnmt1); Growth factor receptor-binding protein 10 (Grb10)
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