Study on effect and mechanism of essential garlic oil in inhibiting monocyte-vascular endothelial cell adhesion induced by interleukin-1α

Abstract

To observe the effects of essential garlic oil (EGO) on vascular cell adhesive molecule-1 (VCAM-1) expression of endothelial cell and monocyte adhesion induced by interleukin-1 alpha (IL-1 alpha). Human umbilical vein endothelial cells (HUVEC) were isolated by pancreatin digestion method and co-cultured with U937 monocyte and IL-1 alpha or EGO + IL-1 alpha respectively. The adhesion rate of monocyte to endothelial cell was measured and mean fluorescent intensity of immuno-stained of VCAM-1 was determined by ACAS 570. EGO showed significant inhibition on VCAM-1 expression induced by IL-1 alpha either in simple culture with HUVEC or in co-culture with HUVEC + U937, and could obviously down regulate the monocyte-endothelial cell adhesion under effect of IL-1 alpha. EGO has the effect of antagonizing against adhesion of monocyte and vascular endothelial cell, it may be due to the inhibition of EGO on adhesive molecular expression on endothelial cell.