Study on Distribution Behavior in Aqueous Two-phase System about Four Kinds of Alkaloids in Coptis Chinensis

Abstract

Using aqueous two-phase to extract alkaloids in coptis chinensis. By changing the aqueous two-phase systems (molecular weight and concentration of polyethylene glycol (PEG), kinds and concentration of salt), and researched the distribution behavior of four major alkaloids in rhizoma coptidis: berberine, coptisine, palmatine and jatrorrhizin in aqueous two-phase system. Combined with ultraviolet Spectrophotometric to detect the extraction. Found that the four alkaloids extraction from high to low as follows: berberine, palmatine, jatrorrhizin and coptisine. The extraction rate of them is more than 98%. Hydrophobic interaction is one of the main factors, which directly affects the extraction of aqueous two-phase system.