Abstract

To understand the detoxification effect of biochemical fulvic acid (BFA) on Quasipaa boulengeri (Q.boulengeri ) tadpoles under copper stress, effects of different concentrations of BFA on growth and physiological indexes of tadpoles. The results showed that the survival rate of tadpoles increased with the increase of BFA concentration. With the increase of BFA concentration, the growth rate of body weight and body length reached the peak at 45 mg/L; The superoxide dismutase(SOD) showed a downward trend in the control group (CK) and an upward trend in each treatment group, with significant difference between the treatment groups (P < 0.05); The content of the malondialdehyde(MDA) and the treatment time and concentration of BFA showed an upward trend in CK group. With the increase of BFA concentration and the content of the MDA decreased, there was significant difference between the treatment groups (P < 0.05). In conclusion, BFA can be used as a detoxification agent under copper stress, and the suitable concentration of BFA is 45 mg/L. This study provides a theoretical basis for the artificial breeding and scientific protection of Q.boulengeri tadpoles.

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