Abstract
Objective To detect the endothelial function of external iliac artery in rats with different stages of atherosclerosis by high-resolution ultrasound, so as to provide experimental methodological basis for evaluating the function of vascular endothelial cells by ultrasound. Methods The animals were randomly divided into the control group (n = 6) and the atherosclerosis model group (n = 15). The atherosclerosis group was further divided into 4-week group, 8-week group, and 12-week group, with 5 animals in each group. After separating and grinding rat spleen, the obtained cells were cultured by density gradient centrifugation. After the cells adhered, the morphology of the cells was observed under a microscope and identified by DiI-Ac-LDL and FITC-UEA-I double staining. The activities of LDH and SOD, the contents of MDA and GSH, and the contents of NO in plasma were detected by biochemical methods. Results The protective effect of rosanilin on brain injury in rats with acute hypobaric hypoxia and its regulation on the expression of pAkt protein; ox-LDL inhibited the proliferation activity of EPCs in a concentration-dependent manner. The expression of KLF2 and S1PR1 in HAEC can be knocked down by small interfering RNA, and knocking down KLF2 can not only downregulate the expression of S1PR1 but also downregulate HAVEN. With the development of atherosclerosis, the endothelium-dependent relaxation function and endothelium-independent relaxation function of the control group and the atherosclerosis model at 4, 8, and 12 weeks were damaged in different degrees and gradually aggravated. Conclusion Atherosclerosis is a disease with both morphological and functional damage, and vascular endothelial function is damaged in the early stage with corresponding pathological changes. Ultrasound is an effective method to evaluate vascular endothelial function.
Highlights
Vascular endothelial cells have physiological barrier function and have extremely active metabolic function
Patients with coronary heart disease are often accompanied by endothelium-dependent dilation disorder of brachial artery and coronary artery, which suggests that these two sites are interrelated and that endothelial dysfunction is a systematic disease in atherosclerosis
After 18 hours of acute hypobaric hypoxia, there was obvious separation between the pia mater and the cerebral cortex, and a large number of red-stained red blood cells could be seen in the gap
Summary
Vascular endothelial cells have physiological barrier function and have extremely active metabolic function. Exploring the factors and mechanism of vascular endothelial cell injury has become the focus of cardiovascular research. The methods used are mainly based on the diastolic dysfunction caused by endothelial cell injury. Ultrasonic cavitation can damage the vessel wall, increase the permeability of the vessel wall, shed endothelial cells, rupture and bleed the vessel wall, and form thrombus and other biological effects, which have been confirmed in the study of rat ear vein, myocardium, BioMed Research International mesentery, spleen microvessels, and various tumor tissue microvessels [1]. When vascular endothelium is damaged, active substances change, vasoconstriction spasm, platelet self-coagulation, smooth muscle cell proliferation, and vascular remodeling, that is, vascular endothelial dysfunction. It is possible to find microscopic lesions from macroscopic imaging by using the interaction of microbubbles with endothelial cells and leukocytes and their backscattering characteristics
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
