Study on continuous (254 nm) and pulsed UV (266 and 355 nm) lights on BVD virus inactivation and its effects on biological properties of fetal bovine serum

Abstract

Both continuous UV lights and pulsed UV lasers have potentials to inactivate known and emerging viruses. Bovine viral diarrhea virus (BVDV), from the Pestivirus genus, is known to be a common viral contamination in (fetal) bovine serum (FBS). Also, BVDV has been used in the blood product industry as a surrogate for Hepatitis C virus (HCV), due to its similarity in structure and genome. Germicidal UV lamp with the wavelength of 254 nm and Nd:YAG laser (pulsed UV laser) in its third and fourth harmonic with the wavelengths of 355 and 266 nm, respectively, were used. BVDV suspended in PBS or FBS were exposed to different intensities and doses and then reduction in BVDV titer were calculated. To complete inactivation of BVDV suspended in PBS and PBS containing 5% FBS, 1.6 ( t = 30 min) and 3.2 ( t = 60 min) J/cm 2 were used. The minimum doses for inactivation of BVDV suspended in PBS with the 355 and 266 nm of pulsed UV laser were 352 and 92.25 J/cm 2. Also, the minimum doses for inactivation of BVDV suspended in FBS with 355 and 266 nm wavelengths of pulsed UV laser were 704 and 127 J/cm 2. To evaluate the irradiated FBS quality to support cell culture growth, FBS was treated with the dose of 190.5 J/cm 2 and 266 nm pulsed UV laser and was used to grow Vero cells, in comparison with a control group. The viability of cells in two groups was identical and the statistical evaluation showed no significant difference in 12 passages.